RT Journal Article SR Electronic(1) A1 Melanson, Rebecca A A1 Barphagha, Inderjit A1 Osti, Surendra A1 Lelis, Tiago P A1 Karki, Hari S A1 Chen, Ruoxi A1 Shrestha, Bishnu K A1 Ham, Jong HyunYR 2017 T1 Identification of new regulatory genes involved in the pathogenic functions of the rice-pathogenic bacterium Burkholderia glumae JF Microbiology, VO 163 IS 2 SP 266 OP 279 DO https://doi.org/10.1099/mic.0.000419 PB Microbiology Society, SN 1465-2080, AB Burkholderia glumae is an emerging plant-pathogenic bacterium that causes disease in rice in several of the major rice-producing areas throughout the world. In the southern United States, B. glumae is the major causal agent of bacterial panicle blight of rice and has caused severe yield losses in recent decades. Despite its importance, few management options are available for diseases caused by B. glumae, and knowledge of how this pathogen causes disease is limited. In an effort to identify novel factors that contribute to the pathogenicity of B. glumae, random mutagenesis using the miniTn5gus transposon was performed on two strains of B. glumae. Resultant mutants were screened in the laboratory for altered phenotypes in various known or putative virulence factors, including toxoflavin, lipase and extracellular polysaccharides. Mutants that exhibited altered phenotypes compared to their parent strain were selected and subsequently characterized using a PCR-based method to identify the approximate location of the transposon insertion. Altogether, approximately 20 000 random mutants were screened and 51 different genes were identified as having potential involvement in the production of toxoflavin, lipase and/or extracellular polysaccharide. Especially, two regulatory genes, ntpR and tepR, encoding a LysR-type transcriptional regulator and a σ54-dependent response regulator, respectively, were discovered in this study as new negative regulatory factors for the production of toxoflavin, the major phytotoxin synthesized by B. glumae and involved in bacterial pathogenesis., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000419