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Volume 163, Issue 2

Suppressor analysis of mutant defects reveals coordinate regulation of secondary metabolites and serralysin biosynthesis by EepR and HexS Free

Abstract

The EepR transcription factor positively regulates secondary metabolites and tissue-damaging metalloproteases. To gain insight into mechanisms by which EepR regulates pigment and co-regulated factors, genetic suppressor analysis was performed. Suppressor mutations that restored pigment to the non-pigmented ∆ mutant mapped to the ORF. Mutation of also restored haemolysis, swarming motility and protease production to the mutant. HexS is a known direct and negative regulator of secondary metabolites in and is a LysR family regulator and an orthologue of LrhA. Here, we demonstrate that HexS directly controls and the serralysin gene . EepR was shown to directly regulate expression but indirectly regulate expression. Together, these data indicate that EepR and HexS oppose each other in controlling stationary phase-associated molecules and enzymes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biosurfactant , motility , prodigiosin , secondary metabolism and transcriptional regulation
© 2017 The Authors
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2017-02-01
2024-04-19
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Suppressor analysis of eepR mutant defects reveals coordinate regulation of secondary metabolites and serralysin biosynthesis by EepR and HexS
Microbiology 163, 280 (2017); https://doi.org/10.1099/mic.0.000422
/content/journal/micro/10.1099/mic.0.000422
/content/journal/micro/10.1099/mic.0.000422
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