RT Journal Article SR Electronic(1) A1 Nazik, Hasan A1 Joubert, Lydia-Marie A1 Secor, Patrick R. A1 Sweere, Johanna M. A1 Bollyky, Paul L. A1 Sass, Gabriele A1 Cegelski, Lynette A1 Stevens, David A.YR 2017 T1 Pseudomonas phage inhibition of Candida albicans JF Microbiology, VO 163 IS 11 SP 1568 OP 1577 DO https://doi.org/10.1099/mic.0.000539 PB Microbiology Society, SN 1465-2080, AB Pseudomonas aeruginosa (Pa) and Candida albicans (Ca) are major bacterial and fungal pathogens in immunocompromised hosts, and notably in the airways of cystic fibrosis patients. The bacteriophages of Pa physically alter biofilms, and were recently shown to inhibit the biofilms of Aspergillus fumigatus. To understand the range of this viral–fungal interaction, we studied Pa phages Pf4 and Pf1, and their interactions with Ca biofilm formation and preformed Ca biofilm. Both forms of Ca biofilm development, as well as planktonic Ca growth, were inhibited by either phage. The inhibition of biofilm was reversed by the addition of iron, suggesting that the mechanism of phage action on Ca involves denial of iron. Birefringence studies on added phage showed an ordered structure of binding to Ca. Electron microscopic observations indicated phage aggregation in the biofilm extracellular matrix. Bacteriophage–fungal interactions may be a general feature with several pathogens in the fungal kingdom., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000539