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Volume 163, Issue 11

The amino-terminal domain of ELL transcription elongation factor is essential for ELL function in Free

Abstract

Transcriptional elongation is a critical step for regulating expression of protein-coding genes. Multiple transcription elongation factors have been identified , but the physiological roles of many of them are still not clearly understood. The ELL (Eleven nineteen Lysine rich Leukemia) family of transcription elongation factors are conserved from fission yeast to humans. contains a single ELL homolog (SpELL) that is not essential for its survival. Therefore to gain insights into the cellular functions of SpELL, we identified phenotypes associated with deletion of in . Our results demonstrate that SpELL is required for normal growth of cells. Furthermore, cells lacking exhibit a decrease in survival when exposed to DNA-damaging conditions, but their growth is not affected under environmental stress conditions. ELL orthologs in different organisms contain three conserved domains, an amino-terminal domain, a middle domain and a carboxyl-terminal domain. We also carried out an functional mapping of these conserved domains within ELL and uncovered a critical role for its amino-terminus in regulating all its cellular functions, including growth under different conditions, transcriptional elongation potential and interaction with EAF. Taken together our results suggest that the domain organization of ELL proteins is conserved across species, but the functions as well as the relationship between the various domains and roles of ELL show species-specific differences.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): EAF , ELL , gene regulation , RNA polymerase II , S. pombe and transcriptional elongation
© 2017 The Authors
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2017-11-01
2024-04-16
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The amino-terminal domain of ELL transcription elongation factor is essential for ELL function in Schizosaccharomyces pombe
Microbiology 163, 1641 (2017); https://doi.org/10.1099/mic.0.000554
/content/journal/micro/10.1099/mic.0.000554
/content/journal/micro/10.1099/mic.0.000554
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