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Volume 163, Issue 11

Unravelling the transcriptional regulation of genes: the dual role of transcription factor Leu3 Free

Abstract

Yeast cells can use γ-aminobutyric acid (GABA), a non-protein amino acid, as a nitrogen source that is mainly imported by the permease Uga4 and catabolized by the enzymes GABA transaminase and succinate-semialdehyde dehydrogenase, encoded by the and genes, respectively. The three genes are inducible by GABA and subject to nitrogen catabolite repression. Hence, their regulation occurs through two mechanisms, one dependent on the inducer and the other on nitrogen source quality. The aim of this work was to better understand the molecular mechanisms of transcription factors acting on different regulatory elements present in promoters, such as Uga3, Dal81, Leu3 and the GATA factors, and to establish the mechanism of the concerted action between them. We found that Gat1 plays an important role in the induction of transcription by GABA and that Gzf3 has an effect in cells grown in a poor nitrogen source such as proline and that this effect is positive on expression. We also found that Gln3 and Dal80 affect the interaction of Uga3 and Dal81 on promoters. Moreover, our results indicated that the repressing activity of Leu3 on and occurs through Dal80 since we demonstrated that Leu3 facilitates Dal80 interaction with DNA. However, when the expression of GATA factors is null or negligible, Leu3 functions as an activator.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): GABA , GATA factors , Leu3 , Saccharomyces cerevisiae , transcription and UGA genes
© 2017 The Authors
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2017-11-01
2024-04-19
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Unravelling the transcriptional regulation of Saccharomyces cerevisiae UGA genes: the dual role of transcription factor Leu3
Microbiology 163, 1692 (2017); https://doi.org/10.1099/mic.0.000560
/content/journal/micro/10.1099/mic.0.000560
/content/journal/micro/10.1099/mic.0.000560
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