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Volume 163, Issue 12

Ethylene production in sp. PCC 6803 promotes phototactic movement Free

Abstract

Ethylene is a gaseous signal sensed by plants and bacteria. Heterologous expression of the ethylene-forming enzyme (EFE) from in cyanobacteria leads to the production of ethylene under photoautotrophic conditions. The recent characterization of an ethylene-responsive signalling pathway affecting phototaxis in the cyanobacterium sp. PCC 6803 implied that biotechnologically relevant ethylene synthesis may induce regulatory processes that are not related to changes in metabolism. Here, we provide data that indicate that endogenously produced ethylene accelerates the movement of cells towards light. Microarray analysis demonstrates that ethylene mainly deactivates transcription from the promoter, which is under the control of the two-component system consisting of the ethylene- and UV-A-sensing histidine kinase UirS and the DNA-binding response regulator UirR. Surprisingly, ethylene production triggers a very specific transcriptional response and only a few other smaller transcriptional changes are detected in the microarray analysis.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cyanobacteria , ethylene , phototaxis and transcription
© 2017 The Authors
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2017-12-01
2024-04-19
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Ethylene production in Synechocystis sp. PCC 6803 promotes phototactic movement
Microbiology 163, 1937 (2017); https://doi.org/10.1099/mic.0.000564
/content/journal/micro/10.1099/mic.0.000564
/content/journal/micro/10.1099/mic.0.000564
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