1887

Abstract

Metallothioneins (MTs) are small proteins with highly conserved cysteine residues and are involved in metal homeostasis and metal detoxification. Two metallothionein genes and from the ectomycorrhizal fungus were characterised for their potential role in heavy metal detoxification. The response of these MTs to the exogenous concentrations of copper and cadmium was studied by qPCR analysis. The exogenous copper but not the cadmium at the tested concentrations induced the expression of the MT genes. The functional role of was validated by expressing the two genes through functional complementation in yeast mutant strain (copper-sensitive), (cadmium- sensitive) and (zinc-sensitive). The mutant strain successfully expressed the two genes resulting in wild-type phenotype restoration of copper, cadmium and zinc tolerance. The present study shows that the ectomycorrhizal fungus encodes two metallothionein genes ( and ) which are more inducible by copper than cadmium and could play an important role in their detoxification.

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2018-06-01
2024-03-28
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