RT Journal Article SR Electronic(1) A1 Hughes, Rebecca-Ayme A1 Jin, Xiaohe A1 Zhang, Yunlong A1 Zhang, Ran A1 Tran, Sabrina A1 Williams, Philip G. A1 Lindsey, Jonathan S. A1 Miller, Eric S.YR 2018 T1 Genome sequence, metabolic properties and cyanobacterial attachment of Porphyrobacter sp. HT-58-2 isolated from a filamentous cyanobacterium–microbial consortium JF Microbiology, VO 164 IS 10 SP 1229 OP 1239 DO https://doi.org/10.1099/mic.0.000706 PB Microbiology Society, SN 1465-2080, AB Tolyporphins are structurally diverse tetrapyrrole macrocycles produced by the cyanobacterial culture HT-58-2. Although tolyporphins were discovered over 25 years ago, little was known about the microbiology of the culture. The studies reported herein expand the description of the community of predominantly alphaproteobacteria associated with the filamentous HT-58-2 cyanobacterium and isolate a dominant bacterium, Porphyrobacter sp. HT-58-2, for which the complete genome is established and growth properties are examined. Fluorescence in situ hybridization (FISH) analysis of the cyanobacterium–microbial community with a probe targeting the 16S rRNA of Porphyrobacter sp. HT-58-2 showed fluorescence emanating from the cyanobacterial sheath. Although genes for the biosynthesis of bacteriochlorophyll a (BChl a) are present in the Porphyrobacter sp. HT-58-2 genome, the pigment was not detected under the conditions examined, implying the absence of phototrophic growth. Comparative analysis of four Porphyrobacter spp. genomes from worldwide collection sites showed significant collinear gene blocks, with two inversions and three deletion regions. Taken together, the results enrich our understanding of the HT-58-2 cyanobacterium–microbial culture., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000706