@article{mbs:/content/journal/micro/10.1099/mic.0.000710, author = "Shashkov, Alexander S. and Kenyon, Johanna J. and Arbatsky, Nikolay P. and Shneider, Mikhail M. and Popova, Anastasiya V. and Knirel, Yuriy A. and Hall, Ruth M.", title = "Genetics of biosynthesis and structure of the K53 capsular polysaccharide of Acinetobacter baumannii D23 made up of a disaccharide K unit", journal= "Microbiology", year = "2018", volume = "164", number = "10", pages = "1289-1292", doi = "https://doi.org/10.1099/mic.0.000710", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000710", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Acinetobacter baumannii", keywords = "K locus", keywords = "2,4-diacetamido-2,4,6-trideoxy-D-glucose", keywords = "KL53", keywords = "capsular polysaccharide", keywords = "2-acetamido-2-deoxy-d-galacturonic acid", abstract = "The KL53 capsular polysaccharide (CPS) gene cluster of Acinetobacter baumannii D23 was sequenced, and includes a single gtr gene encoding the glycosyltransferase Gtr2, and the itrA1 gene for ItrA1 that is known to initiate CPS biosynthesis with d-QuiNAc4NAc. The K53 CPS was isolated and studied by one- and two-dimensional 1H and 13C nuclear magnetic resonance (NMR) spectroscopy before and after O-deacetylation. The disaccharide K unit of the CPS was established as →3)-α-d-GalpNAcA4Ac-(1→3)-β-d-QuipNAc4NAc-(1→, where GalNAcA and QuiNAc4NAc indicate 2-acetamido-2-deoxygalacturonic acid and 2,4-diacetamido-2,4,6-trideoxyglucose, respectively. This established the linkage formed by Gtr2. The degree of 4-O-acetylation of d-GalNAcA by Atr18, encoded at the KL53 locus, is ~55 %.", }