1887

Abstract

The transcriptional regulation of three distinct alcohol oxidation systems, alcohol dehydrogenase (ADH)-I, ADH-IIB and ADH-IIG, in HK5 was investigated under various induction conditions. The promoter activities of the genes involved in alcohol oxidation were determined using a transcriptional fusion promoter-probe vector. Ethanol was the best inducer for the divergent promoters of and , encoding ADH-I and a cytochrome , respectively. Primary and secondary C3 and C4 alcohols and butyraldehyde specifically induced the divergent promoters of and , encoding ADH-IIB and an NAD-dependent aldehyde dehydrogenase, respectively. The promoter of ADH-IIG responded well to ()-(+)-1,2-propanediol induction. In addition, the roles of genes encoding the response regulators and , located downstream of were inferred from the properties of - or -disrupted mutants and gene complementation tests. The gene products of both and were strictly necessary for transcription. The mutation and complementation studies also suggested a role for AgmR, but not ExaE, in the transcriptional regulation of (ADH-IIB) and (AGH-IIG). A hypothetical scheme describing a regulatory network, which directs expression of the three distinct alcohol oxidation systems in HK5, was derived.

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2009-02-01
2024-03-28
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