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Volume 155, Issue 3

Immune evasion by conferred by iron-regulated surface determinant protein IsdH Free

Abstract

The ability of to avoid innate immune responses including neutrophil-mediated phagocytosis is crucial for the organism to cause infection. This multifactorial process involves several secreted and cell-surface-associated proteins. In this paper we report a novel mechanism of combating neutrophils that involves iron-regulated surface determinant protein H (IsdH). The IsdH protein is part of a complex that is only expressed under iron-restricted conditions in order to bind haemoglobin and extract and transport haem into the cytoplasm. A null mutant defective in expression of IsdH, and mutants expressing variants of IsdH with substitutions in residues predicted to be involved in ligand binding, were generated from 8325-4. The IsdH-defective mutants were shown by several measures to have reduced virulence compared with the wild-type. The mutant was engulfed more rapidly by human neutrophils in the presence of serum opsonins, survived poorly in fresh whole human blood and was less virulent in a mouse model of sepsis. The protective mechanism seems to stem from an accelerated degradation of the serum opsonin C3b.

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Keyword(s): GST, glutathione S-transferase , Hp–Hb, haptoglobin–haemoglobin , HRP, horseradish peroxidase , NEAT motif, NEAr Transporter motif , NHS, normal human serum and PMNL, polymorphonuclear leukocytes
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2009-03-01
2024-04-16
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Immune evasion by Staphylococcus aureus conferred by iron-regulated surface determinant protein IsdH
Microbiology 155, 667 (2009); https://doi.org/10.1099/mic.0.025684-0
/content/journal/micro/10.1099/mic.0.025684-0
/content/journal/micro/10.1099/mic.0.025684-0
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