1887

Abstract

Bacteria belonging to the genus are ubiquitous in soil and water. Only a few species, including , and the unnamed genomic species (gen. sp.) 3 and 13TU, which together with the soil organism are combined in the (Acb) complex, have been recognized as important nosocomial infectious agents. The ecology, epidemiology and pathology of most species are not yet well established. Lack of practical and accurate methods limits routine identification of clinical isolates and thus hampers precise identification of those of the Acb complex and other species of possible clinical significance. We previously identified a 350 bp highly variable zone on the gene which appeared to be a promising target for rapid molecular identification. In the present study, we validated this method for accuracy on a collection of reference strains belonging to (5 strains), gen. sp. 3 (29 strains), gen. sp. 13TU (18 strains), (30 strains) and one strain each of , . gen. sp. 15TU, . gen. sp. 10, . gen. sp. 11, . gen. sp. ‘between 1 and 3’ and . gen. sp. 14TU=13BJ. This represents the largest analysis to date that compares a large number of well-identified strains of the Acb complex to assess the intra- and interspecies variation within this complex. All were correctly identified with 98.9–100 % intraspecies relatedness based on partial sequence analysis. We then applied this tool to identify 99 clinical isolates from four public hospitals in Marseille, France. All isolates could easily be identified to species as they were separated into 13 species sequence types with a sequence variance of 0–2.6 % from their respective type strains. Of these 99 isolates, 10 were , 52 were , 27 were gen. sp. 3, 5 were , 1 was , and 1 was gen. sp. 13TU. Three were provisionally identified as gen. sp. 9. This is the first work to identify all specimens of a set of clinical isolates at species level using sequence analysis. Our data emphasize the recognition of as an emerging cause of -related infection and confirm that gen. sp. 3 is the second most commonly isolated species after in patients.

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2009-07-01
2024-03-29
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