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Volume 155, Issue 5

An alternative methionine aminopeptidase, MAP-A, is required for nitrogen starvation and high-light acclimation in the cyanobacterium sp. PCC 6803 Free

Abstract

Methionine aminopeptidases (MetAPs or MAPs, encoded by genes) are ubiquitous and pivotal enzymes for protein maturation in all living organisms. Whereas most bacteria harbour only one gene, many cyanobacterial genomes contain two paralogues, the genome of sp. PCC 6803 even three. The physiological function of multiple paralogues remains elusive so far. This communication reports for the first time differential MetAP function in a cyanobacterium. In sp. PCC 6803, the universally conserved gene () is predominantly expressed in exponentially growing cells and appears to be a housekeeping gene. By contrast, expression of () and () genes increases during stress conditions. The paralogue is only transiently expressed, whereas the widely distributed gene appears to be the major MetAP during stress conditions. A -deficient mutant shows a subtle impairment of photosystem II properties even under non-stressed conditions. In particular, the binding site for the quinone Q is affected, indicating specific N-terminal methionine processing requirements of photosystem II components. MAP-A-specific processing becomes essential under certain stress conditions, since the -deficient mutant is severely impaired in surviving conditions of prolonged nitrogen starvation and high light exposure.

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Chla, chlorophyll a , DCBQ, 2,6-dichloro-p-benzoquinone , DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea , MetAP, methionine aminopeptidase , MmapA, mapA mutant , N-terminal methionine excision , NME , PAM, pulse amplitude modulated , PQQ, phenyl-p-quinone , PSII, photosystem II and RACE, rapid amplification of 5′ cDNA ends
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2009-05-01
2024-04-24
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An alternative methionine aminopeptidase, MAP-A, is required for nitrogen starvation and high-light acclimation in the cyanobacterium Synechocystis sp. PCC 6803
Microbiology 155, 1427 (2009); https://doi.org/10.1099/mic.0.026351-0
/content/journal/micro/10.1099/mic.0.026351-0
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