@article{mbs:/content/journal/micro/10.1099/mic.0.026823-0, author = "Champion, Olivia L. and Cooper, Ian A. M. and James, Sarah L. and Ford, Donna and Karlyshev, Andrey and Wren, Brendan W. and Duffield, Melanie and Oyston, Petra C. F. and Titball, Richard W.", title = "Galleria mellonella as an alternative infection model for Yersinia pseudotuberculosis", journal= "Microbiology", year = "2009", volume = "155", number = "5", pages = "1516-1522", doi = "https://doi.org/10.1099/mic.0.026823-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.026823-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "CI, competitive index", keywords = "i.v., intravenous(ly)", keywords = "SOD, superoxide dismutase", abstract = "We report that larvae of the wax moth (Galleria mellonella) are susceptible to infection with the human enteropathogen Yersinia pseudotuberculosis at 37 °C. Confocal microscopy demonstrated that in the initial stages of infection the bacteria were taken up into haemocytes. To evaluate the utility of this model for screening Y. pseudotuberculosis mutants we constructed and tested a superoxide dismutase C (sodC) mutant. This mutant showed increased susceptibility to superoxide, a key mechanism of killing in insect haemocytes and mammalian phagocytes. It showed reduced virulence in the murine yersiniosis infection model and in contrast to the wild-type strain IP32953 was unable to kill G. mellonella. The complemented mutant regained all phenotypic properties associated with SodC, confirming the important role of this metalloenzyme in two Y. pseudotuberculosis infection models.", }