1887

Abstract

EDIN-B (epidermal cell differentiation inhibitor-B; also termed C3Stau) is an exotoxin of which ADP-ribosylates and inactivates Rho GTP binding proteins. The EDIN-B gene () and the gene for exfoliative toxin D () make up the central part of a recently described pathogenicity island. Here we evaluated the prevalence and genetic organization of the pathogenicity island in invasive isolates, and characterized transcription and EDIN-B production using artificial constructs transduced in strains RN6390 and Newman. We found that eight out of121 (7 %) blood culture isolates harbour , which is organized in three novel variants of the original pathogenicity island. In the serum of patients infected with -positive , significant titres of anti-EDIN-B antibodies could be detected. Regulation of transcription depended on the but not on the regulatory system. Furthermore, retrieval of EDIN-B protein secreted by RN6390 required the presence of 2-macroglobulin to inhibit the activity of extracellular proteases. These data suggest that the EDIN-B toxin is produced during human infection, is part of a highly variable pathogenicity island and can be controlled by the gene regulon and secreted bacterial proteases.

Keyword(s): CC, clonal complex
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2010-03-01
2024-03-28
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