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Volume 155, Issue 10

Small heat-shock protein HspL is induced by VirB protein(s) and promotes VirB/D4-mediated DNA transfer in Free

Abstract

is a Gram-negative plant-pathogenic bacterium that causes crown gall disease by transferring and integrating its transferred DNA (T-DNA) into the host genome. We characterized the chromosomally encoded alpha-crystallin-type small heat-shock protein (-Hsp) HspL, which was induced by the virulence () gene inducer acetosyringone (AS). The transcription of but not three other -Hsp genes (, , ) was upregulated by AS. Further expression analysis in various mutants suggested that AS-induced transcription is not directly activated by the VirG response regulator but rather depends on the expression of VirG-activated genes encoding components of the type IV secretion system (T4SS). Among the 11 genes encoded by the operon, HspL protein levels were reduced in strains with deletions of , or . VirB protein accumulation but not transcription levels were reduced in an deletion mutant early after AS induction, implying that HspL may affect the stability of individual VirB proteins or of the T4S complex directly or indirectly. Tumorigenesis efficiency and the VirB/D4-mediated conjugal transfer of an IncQ plasmid RSF1010 derivative between strains were reduced in the absence of HspL. In conclusion, increased HspL abundance is triggered in response to certain VirB protein(s) and plays a role in optimal VirB protein accumulation, VirB/D4-mediated DNA transfer and tumorigenesis.

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Keyword(s): AS, acetosyringone , RFU, relative fluorescence units , T-DNA, transferred DNA , T4SS, type IV secretion system , Ti, tumour-inducing (plasmid) and α-Hsp, α-crystallin-type small heat-shock protein
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2009-10-01
2024-04-20
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Small heat-shock protein HspL is induced by VirB protein(s) and promotes VirB/D4-mediated DNA transfer in Agrobacterium tumefaciens
Microbiology 155, 3270 (2009); https://doi.org/10.1099/mic.0.030676-0
/content/journal/micro/10.1099/mic.0.030676-0
/content/journal/micro/10.1099/mic.0.030676-0
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