1887

Abstract

The prokaryotic cytoskeletal protein MreB is thought to govern cell shape by positioning the cell wall synthetic apparatus at growth sites in the cell. In rod-shaped bacteria it forms helical filaments that run around the periphery of the rod during elongation. Gram-positive bacteria often contain more than one gene. has three -like genes, , and , the first two of which have been shown to be essential under normal growth conditions. Expression of an homologue from the closely related organism did not have any effect on cell growth or morphology. In contrast, expression of from the phylogenetically more distant bacterium produced shape defects and ultimately cell death, due to disruption of the endogenous MreB cytoskeleton. However, expression of either () or () was sufficient to confer a rod shape to deleted for the three isologues, supporting the idea that the three proteins have largely redundant functions in cell morphogenesis. Expression of could fully compensate for the loss of in and led to the formation of rod-shaped cells. In contrast, expression of was not sufficient to confer a rod shape to Δ, indicating that a complex of these three cell shape determinants is not enough for cell morphogenesis of .

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2009-11-01
2024-03-28
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