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Volume 155, Issue 11

Evaluation of the type I signal peptidase as antibacterial target for biofilm-associated infections of Free

Abstract

The development of antibacterial resistance is inevitable and is a major concern in hospitals and communities. Moreover, biofilm-grown bacteria are less sensitive to antimicrobial treatment. In this respect, the Gram-positive is an important source of nosocomial biofilm-associated infections. In the search for new antibacterial therapies, the type I signal peptidase (SPase I) serves as a potential target for development of antibacterials with a novel mode of action. This enzyme cleaves off the signal peptide from secreted proteins, making it essential for protein secretion, and hence for bacterial cell viability. encodes three putative SPases I (denoted Sip1, Sip2 and Sip3), of which Sip1 lacks the catalytic lysine. In this report, we investigated the active SPases I in more detail. Sip2 and Sip3 were found to complement a temperature-sensitive mutant, demonstrating their functional activity. functional activity of purified Sip2 and Sip3 proteins and inhibition of their activity by the SPase I inhibitor arylomycin A were further illustrated using a fluorescence resonance energy transfer (FRET)-based assay. Furthermore, we demonstrated that SPase I not only is an attractive target for development of novel antibacterials against free-living bacteria, but also is a feasible target for biofilm-associated infections.

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Keyword(s): CLSM, confocal laser scanning microscopy , CV, crystal violet , FRET, fluorescence resonance energy transfer and SPase I/Sip, type I signal peptidase
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2009-11-01
2024-04-19
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Evaluation of the type I signal peptidase as antibacterial target for biofilm-associated infections of Staphylococcus epidermidis
Microbiology 155, 3719 (2009); https://doi.org/10.1099/mic.0.031765-0
/content/journal/micro/10.1099/mic.0.031765-0
/content/journal/micro/10.1099/mic.0.031765-0
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