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Volume 155, Issue 12

DsbL and DsbI contribute to periplasmic disulfide bond formation in serovar Typhimurium Free

Abstract

Disulfide bond formation in periplasmic proteins is catalysed by the DsbA/DsbB system in most Gram-negative bacteria. serovar Typhimurium also encodes a paralogous pair of proteins to DsbA and DsbB, DsbL and DsbI, respectively, downstream of a periplasmic arylsulfate sulfotransferase (ASST). We show that DsbL and DsbI function as a redox pair contributing to periplasmic disulfide bond formation and, as such, affect transcription of the pathogenicity island 1 (SPI1) type three secretion system genes and activation of the RcsCDB system, as well as ASST activity. In contrast to DsbA/DsbB, however, the DsbL/DsbI system cannot catalyse the disulfide bond formation required for flagellar assembly. Phylogenic analysis suggests that the genes are ancestral in the , but have been lost in many lineages. Deletion of confers no virulence defect during acute infection of mice.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ASST, arylsulfate sulfotransferase , i.p., intraperitoneal(ly) , SPI1, Salmonella pathogenicity island 1 , T3SS, type III secretion system and UPEC, uropathogenic E. coli
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2009-12-01
2024-04-19
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DsbL and DsbI contribute to periplasmic disulfide bond formation in Salmonella enterica serovar Typhimurium
Microbiology 155, 4014 (2009); https://doi.org/10.1099/mic.0.032904-0
/content/journal/micro/10.1099/mic.0.032904-0
/content/journal/micro/10.1099/mic.0.032904-0
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