@article{mbs:/content/journal/micro/10.1099/mic.0.033977-0, author = "Schwan, William R. and Briska, Adam and Stahl, Buffy and Wagner, Trevor K. and Zentz, Emily and Henkhaus, John and Lovrich, Steven D. and Agger, William A. and Callister, Steven M. and DuChateau, Brian and Dykes, Colin W.", title = "Use of optical mapping to sort uropathogenic Escherichia coli strains into distinct subgroups", journal= "Microbiology", year = "2010", volume = "156", number = "7", pages = "2124-2135", doi = "https://doi.org/10.1099/mic.0.033977-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.033977-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "EHEC, enterohaemorrhagic Escherichia coli", keywords = "UTI, urinary tract infection", keywords = "MLST, multilocus sequence typing", keywords = "UPEC, uropathogenic Escherichia coli", keywords = "PAI, pathogenicity island", keywords = "UPGMA, unweighted pair group method with arithmetic mean", abstract = "Optical maps were generated for 33 uropathogenic Escherichia coli (UPEC) isolates. For individual genomes, the NcoI restriction fragments aligned into a unique chromosome map for each individual isolate, which was then compared with the in silico restriction maps of all of the sequenced E. coli and Shigella strains. All of the UPEC isolates clustered separately from the Shigella strains as well as the laboratory and enterohaemorrhagic E. coli strains. Moreover, the individual strains appeared to cluster into distinct subgroups based on the dendrogram analyses. Phylogenetic grouping of these 33 strains showed that 32/33 were the B2 subgroup and 1/33 was subgroup A. To further characterize the similarities and differences among the 33 isolates, pathogenicity island (PAI), haemolysin and virulence gene comparisons were performed. A strong correlation was observed between individual subgroups and virulence factor genes as well as haemolysis activity. Furthermore, there was considerable conservation of sequenced-strain PAIs in the specific subgroups. Strains with different antibiotic-resistance patterns also appeared to sort into separate subgroups. Thus, the optical maps distinguished the UPEC strains from other E. coli strains and further subdivided the strains into distinct subgroups. This optical mapping procedure holds promise as an alternative way to subgroup all E. coli strains, including those involved in infections outside of the intestinal tract and epidemic strains with distinct patterns of antibiotic resistance.", }