RT Journal Article SR Electronic(1) A1 Sauri, Ana A1 Soprova, Zora A1 Wickström, David A1 de Gier, Jan-Willem A1 Van der Schors, Roel C. A1 Smit, August B. A1 Jong, Wouter S. P. A1 Luirink, JoenYR 2009 T1 The Bam (Omp85) complex is involved in secretion of the autotransporter haemoglobin protease JF Microbiology, VO 155 IS 12 SP 3982 OP 3991 DO https://doi.org/10.1099/mic.0.034991-0 PB Microbiology Society, SN 1465-2080, AB Autotransporters are large virulence factors secreted by Gram-negative bacteria. They are synthesized with a C-terminal domain that forms a β-barrel pore in the outer membrane implicated in translocation of the upstream ‘passenger’ domain across the outer membrane. However, recent structural data suggest that the diameter of the β-barrel pore is not sufficient to allow the passage of partly folded structures observed for several autotransporters. Here, we have used a stalled translocation intermediate of the autotransporter Hbp to identify components involved in insertion and translocation of the protein across the outer membrane. At this intermediate stage the β-domain was not inserted and folded as an integral β-barrel in the outer membrane whereas part of the passenger was surface exposed. The intermediate was copurified with the periplasmic chaperone SurA and subunits of the Bam (Omp85) complex that catalyse the insertion and assembly of outer-membrane proteins. The data suggest a critical role for this general machinery in the translocation of autotransporters across the outer membrane., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.034991-0