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Volume 156, Issue 9

subspecies serovar Enteritidis pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread Free

Abstract

subspecies serovar Enteritidis (. Enteritidis) has been identified as a significant cause of salmonellosis in humans. pathogenicity islands 1 and 2 (SPI-1 and SPI-2) each encode a specialized type III secretion system (T3SS) that enables to manipulate host cells at various stages of the invasion/infection process. For the purposes of our studies we used a chicken isolate of Enteritidis (Sal18). In one study, we orally co-challenged 35-day-old specific pathogen-free (SPF) chickens with two bacterial strains per group. The control group received two versions of the wild-type strain Sal18: Sal18 Tn : :  and Sal18 Tn : : , while the other two groups received the wild-type strain (Sal18 Tn : : ) and one of two mutant strains. From this study, we concluded that . Enteritidis strains deficient in the SPI-1 and SPI-2 systems were outcompeted by the wild-type strain. In a second study, groups of SPF chickens were challenged at 1 week of age with four different strains: the wild-type strain, and three other strains lacking either one or both of the SPI-1 and SPI-2 regions. On days 1 and 2 post-challenge, we observed a reduced systemic spread of the SPI-2 mutants, but by day 3, the systemic distribution levels of the mutants matched that of the wild-type strain. Based on these two studies, we conclude that the . Enteritidis SPI-2 T3SS facilitates invasion and systemic spread in chickens, although alternative mechanisms for these processes appear to exist.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): SCV, Salmonella-containing vesicle , SPF, specific pathogen-free , SPI, Salmonella pathogenicity island and T3SS, type III secretion system
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2010-09-01
2024-04-19
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Salmonella enterica subspecies enterica serovar Enteritidis Salmonella pathogenicity island 2 type III secretion system: role in intestinal colonization of chickens and systemic spread
Microbiology 156, 2770 (2010); https://doi.org/10.1099/mic.0.038018-0
/content/journal/micro/10.1099/mic.0.038018-0
/content/journal/micro/10.1099/mic.0.038018-0
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