RT Journal Article SR Electronic(1) A1 Candela, Marco A1 Centanni, Manuela A1 Fiori, Jessica A1 Biagi, Elena A1 Turroni, Silvia A1 Orrico, Catia A1 Bergmann, Simone A1 Hammerschmidt, Sven A1 Brigidi, PatriziaYR 2010 T1 DnaK from Bifidobacterium animalis subsp. lactis is a surface-exposed human plasminogen receptor upregulated in response to bile salts JF Microbiology, VO 156 IS 6 SP 1609 OP 1618 DO https://doi.org/10.1099/mic.0.038307-0 PB Microbiology Society, SN 1465-2080, AB Bifidobacterium animalis subsp. lactis lives in the gastrointestinal tract of most mammals, including humans. Recently, for the probiotic strain B. animalis subsp. lactis BI07, a dose-dependent plasminogen-binding activity was demonstrated and five putative plasminogen-binding proteins were identified. Here we investigated the role of surface DnaK as a B. animalis subsp. lactis BI07 plasminogen receptor. DnaK was visualized on the bacterial cell surface by transmission electron microscopy. The His-tagged recombinant DnaK protein showed a high affinity for human plasminogen, with an equilibrium dissociation constant in the nanomolar range. The capability to tolerate physiological concentrations of bile salts is a crucial feature for an intestinal symbiont micro-organism. By proteome analysis we demonstrated that the long-term exposure of B. animalis subsp. lactis BI07 to bile salts results in the upregulation of important surface plasminogen receptors such as DnaK and enolase. Moreover, adaptation of B. animalis subsp. lactis BI07 to physiological concentrations of bile salts significantly increased its capacity to interact with the host plasminogen system. By enhancing the bacterial capacity to interact with the host plasminogen, the gut bile environment may facilitate the colonization of the human host by B. animalis subsp. lactis BI07., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.038307-0