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Volume 156, Issue 6

The high-adhesive properties of the FimH adhesin of serovar Enteritidis are determined by a single F118S substitution Free

Abstract

The binding properties of low- and high-adhesive forms of FimH adhesins from serovars Enteritidis and Typhimurium ( Enteritidis and Typhimurium) were studied using chimeric proteins containing an additional peptide that represents an N-terminal extension of the FimF protein. This modification, by taking advantage of a donor strand exchange mechanism, closes the hydrophobic groove in the fimbrial domain of the FimH adhesin. Such self-complemented adhesins (scFimH) did not form aggregates and were more stable (resistant to proteolytic cleavage) than native FimH. High-adhesive variants of scFimH proteins, with alanine at position 61 and serine at position 118, were obtained by site-directed mutagenesis of genes from low-adhesive variants of Enteritidis and Typhimurium, with glycine at position 61 and phenylalanine at position 118. Direct kinetic analysis using surface plasmon resonance (SPR) and glycoproteins carrying high-mannose carbohydrate chains (RNase B, horseradish peroxidase and mannan-BSA) revealed the existence of high- and low-adhesive allelic variants, not only in Typhimurium but also in Enteritidis. Using two additional mutants of low-adhesive FimH protein from Enteritidis (Gly61Ala and Phe118Ser), SPR analysis pointed to Ser118 as the major determinant of the high-adhesive phenotype of type 1 fimbriae from Enteritidis. These studies demonstrated for the first time that the functional differences observed with whole fimbriated bacteria could be reproduced at the level of purified adhesin. They strongly suggest that the adhesive properties of type 1 fimbriae are determined only by structural differences in the FimH proteins and are not influenced by the fimbrial shaft on which the adhesin is located.

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Keyword(s): CD, circular dichroism , HRP, horseradish peroxidase and SPR, surface plasmon resonance
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2010-06-01
2024-04-19
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The high-adhesive properties of the FimH adhesin of Salmonella enterica serovar Enteritidis are determined by a single F118S substitution
Microbiology 156, 1738 (2010); https://doi.org/10.1099/mic.0.039206-0
/content/journal/micro/10.1099/mic.0.039206-0
/content/journal/micro/10.1099/mic.0.039206-0
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