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Volume 156, Issue 9

Role of polysaccharide () genes in VPS production, biofilm formation and pathogenesis Free

Abstract

Biofilm formation enhances the survival and persistence of the facultative human pathogen in natural ecosystems and its transmission during seasonal cholera outbreaks. A major component of the biofilm matrix is the polysaccharide (VPS), which is essential for development of three-dimensional biofilm structures. The genes are clustered in two regions, the I cluster (, , VC0916–27) and the II cluster (, VC0934–39), separated by an intergenic region containing the gene cluster that encodes biofilm matrix proteins. In-frame deletions of the clusters and genes encoding matrix proteins drastically altered biofilm formation phenotypes. To determine which genes within the gene clusters are required for biofilm formation and VPS synthesis, we generated in-frame deletion mutants for all the genes. Many of these mutants exhibited reduced capacity to produce VPS and biofilms. Infant mouse colonization assays revealed that mutants lacking either clusters or (encoding secreted matrix protein RbmA) exhibited a defect in intestinal colonization compared to the wild-type. Understanding the roles of the various gene products will aid in the biochemical characterization of the VPS biosynthetic pathway and elucidate how gene products contribute to VPS biosynthesis, biofilm formation and virulence in .

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Keyword(s): CSLM, confocal scanning laser microscopy and VPS, Vibro polysaccharide
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2010-09-01
2024-04-18
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Role of Vibrio polysaccharide (vps) genes in VPS production, biofilm formation and Vibrio cholerae pathogenesis
Microbiology 156, 2757 (2010); https://doi.org/10.1099/mic.0.040196-0
/content/journal/micro/10.1099/mic.0.040196-0
/content/journal/micro/10.1099/mic.0.040196-0
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