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Volume 157, Issue 1

Citrus limonoids interfere with cell–cell signalling and biofilm formation by modulating the response regulator LuxO Free

Abstract

Citrus limonoids are unique secondary metabolites, characterized by a triterpenoid skeleton with a furan ring. Studies have demonstrated beneficial health properties of limonoids. In addition, certain citrus limonoids play a role in plant defence against insect pests. In the present study, five limonoids were purified from sour orange and evaluated for their ability to inhibit cell–cell signalling. The purified limonoids were tested for their ability to interfere with cell–cell signalling and biofilm formation in . Isolimonic acid, deacetylnomilinic acid glucoside and ichangin demonstrated significant inhibition of autoinducer-mediated cell–cell signalling and biofilm formation. Furthermore, isolimonic acid and ichangin treatment resulted in induced expression of the response regulator gene . In addition, promoter activity was not affected by isolimonic acid or ichangin. Therefore, the ability of isolimonic acid and ichangin to interfere with cell–cell signalling and biofilm formation seems to stem from the modulation of expression. The results suggest that isolimonic acid and ichangin are potent modulators of bacterial cell–cell signalling.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ACN, acetonitrile , AI-2, autoinducer-2 , APCI, atmospheric pressure chemical ionization , CFS, cell-free culture supernatant , DCM, dichloromethane , DNAG, deacetyl nomilinic acid 17β-d-glucopyranoside , EtOAc, ethyl acetate , HAI, harveyi autoinducer , IOAG, isoobacunoic acid 17β-d-glucopyranoside , MeOH, methanol and qRT-PCR, quantitative RT-PCR
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2011-01-01
2024-04-18
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Citrus limonoids interfere with Vibrio harveyi cell–cell signalling and biofilm formation by modulating the response regulator LuxO
Microbiology 157, 99 (2011); https://doi.org/10.1099/mic.0.041228-0
/content/journal/micro/10.1099/mic.0.041228-0
/content/journal/micro/10.1099/mic.0.041228-0
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