1887

Microbiology Society logo

Volume 156, Issue 12

serine recombinase genes are widely distributed in the and species groups and are expressed in Free

Abstract

The presence, distribution and expression of cassette chromosome recombinase () genes, which are homologous to the staphylococcal genes and are designated genes, were examined in enterococcal isolates (=421) representing 13 different species. A total of 118 (28 %) isolates were positive for genes by PCR, and a number of these were confirmed by Southern hybridization with a probe (=76) and partial DNA sequencing of and genes (=38). genes were present in (58/216, 27 %), (31/38, 82 %), (27/52, 50 %), (1/4, 25 %) and (1/2, 50 %). In the eight other species tested, including (=94), genes were not found. Thirty-eight sequenced genes from five different enterococcal species showed 94–100 % nucleotide sequence identity and linkage PCRs showed heterogeneity in the flanking chromosomal genes. Expression analysis of genes from the DO strain showed constitutive expression as a bicistronic mRNA. The mRNA levels were lower during log phase than stationary phase in relation to total mRNA. Multilocus sequence typing was performed on 39 isolates. genes were detected in both hospital-related (10/29, 34 %) and non-hospital (4/10, 40 %) strains of . Various sequence types were represented by both positive and negative isolates, suggesting acquisition or loss of in . In summary, genes, potentially involved in genome plasticity, are expressed in and are widely distributed in the and species groups.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): CDSs, coding sequences , JGI, Joint Genome Institute , MLST, multilocus sequence type , s: ATCC, American Type Culture Collection , SCC, staphylococcal cassette chromosome and ST, sequence type
SGM
Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.041491-0
2010-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/156/12/3624.html?itemId=/content/journal/micro/10.1099/mic.0.041491-0&mimeType=html&fmt=ahah

References

  1. Arduino R. C., Murray B. E., Rakita R. M. 1994; Roles of antibodies and complement in phagocytic killing of enterococci. Infect Immun 62:987–993
     [Google Scholar]
  2. Baele M., Baele P., Vaneechoutte M., Storms V., Butaye P., Devriese L. A., Verschraegen G., Gillis M., Haesebrouck F. 2000; Application of tRNA intergenic spacer PCR for identification of Enterococcus species. J Clin Microbiol 38:4201–4207
     [Google Scholar]
  3. Besemer J., Borodovsky M. 1999; Heuristic approach to deriving models for gene finding. Nucleic Acids Res 27:3911–3920
     [Google Scholar]
  4. Bruen T. C., Philippe H., Bryant D. 2006; A simple and robust statistical test for detecting the presence of recombination. Genetics 172:2665–2681
     [Google Scholar]
  5. Carias L. L., Rudin S. D., Donskey C. J., Rice L. B. 1998; Genetic linkage and cotransfer of a novel, vanB -containing transposon (Tn 5382 ) and a low-affinity penicillin-binding protein 5 gene in a clinical vancomycin-resistant Enterococcus faecium isolate. J Bacteriol 180:4426–4434
     [Google Scholar]
  6. Dahl K. H., Sundsfjord A. 2003; Transferable vanB2 Tn 5382 -containing elements in fecal streptococcal strains from veal calves. Antimicrob Agents Chemother 47:2579–2583
     [Google Scholar]
  7. Dahl K. H., Simonsen G. S., Olsvik Ø., Sundsfjord A. 1999; Heterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci. Antimicrob Agents Chemother 43:1105–1110
     [Google Scholar]
  8. Devriese L. A., Pot B., Collins M. D. 1993; Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J Appl Bacteriol 75:399–408
     [Google Scholar]
  9. Dutka-Malen S., Evers S., Courvalin P. 1995; Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR. . J Clin Microbiol 33:24–27
     [Google Scholar]
  10. Hanssen A. M., Kjeldsen G., Sollid J. U. E. 2004; Local variants of staphylococcal cassette chromosome mec in sporadic methicillin-resistant Staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci: evidence of horizontal gene transfer?. Antimicrob Agents Chemother 48:285–296
     [Google Scholar]
  11. Homan W. L., Tribe D., Poznanski S., Li M., Hogg G., Spalburg E., van Embden J., Willems R. 2002; Multilocus sequence typing scheme for Enterococcus faecium . J Clin Microbiol 40:1963–1971
     [Google Scholar]
  12. International Working Group on the Classification of Staphylococcal Cassette Chromosome Elements 2009; Classification of staphylococcal cassette chromosome mec (SCC mec ): guidelines for reporting novel SCC mec elements. Antimicrob Agents Chemother 53:4961–4967
     [Google Scholar]
  13. Ito T., Katayama Y., Hiramatsu K. 1999; Cloning and nucleotide sequence determination of the entire mec DNA of pre-methicillin-resistant Staphylococcus aureus N315. Antimicrob Agents Chemother 43:1449–1458
     [Google Scholar]
  14. Ito T., Katayama Y., Asada K., Mori N., Tsutsumimoto K., Tiensasitorn C., Hiramatsu K. 2001; Structural comparison of three types of staphylococcal cassette chromosome mec integrated in the chromosome in methicillin-resistant Staphylococcus aureus . Antimicrob Agents Chemother 45:1323–1336
     [Google Scholar]
  15. Ito T., Ma X. X., Takeuchi F., Okuma K., Yuzawa H., Hiramatsu K. 2004; Novel type V staphylococcal cassette chromosome mec driven by a novel cassette chromosome recombinase, ccrC . Antimicrob Agents Chemother 48:2637–2651
     [Google Scholar]
  16. Johnsen P. J., Østerhus J. I., Sletvold H., Sørum M., Kruse H., Nielsen K., Simonsen G. S., Sundsfjord A. 2005; Persistence of animal and human glycopeptide-resistant enterococci on two Norwegian poultry farms formerly exposed to avoparcin is associated with a widespread plasmid-mediated vanA element within a polyclonal Enterococcus faecium population. Appl Environ Microbiol 71:159–168
     [Google Scholar]
  17. Katayama Y., Ito T., Hiramatsu K. 2000; A new class of genetic element, Staphylococcus cassette chromosome mec , encodes methicillin resistance in Staphylococcus aureus . Antimicrob Agents Chemother 44:1549–1555
     [Google Scholar]
  18. Katayama Y., Takeuchi F., Ito T., Ma X. X., Ui-Mizutani Y., Kobayashi I., Hiramatsu K. 2003; Identification in methicillin-susceptible Staphylococcus hominis of an active primordial mobile genetic element for the staphylococcal cassette chromosome mec of methicillin-resistant Staphylococcus aureus . J Bacteriol 185:2711–2722
     [Google Scholar]
  19. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5:150–163
     [Google Scholar]
  20. Leavis H. L., Bonten M. J., Willems R. J. 2006; Identification of high-risk enterococcal clonal complexes: global dispersion and antibiotic resistance. Curr Opin Microbiol 9:454–460
     [Google Scholar]
  21. Leavis H. L., Willems R. J., van Wamel W. J., Schuren F. H., Caspers M. P., Bonten M. J. 2007; Insertion sequence-driven diversification creates a globally dispersed emerging multiresistant subspecies of E. faecium . PLoS Pathog 3:e7
     [Google Scholar]
  22. Noto M. J., Archer G. L. 2006; A subset of Staphylococcus aureus strains harboring staphylococcal cassette chromosome mec (SCC mec ) type IV is deficient in CcrAB-mediated SCC mec excision. Antimicrob Agents Chemother 50:2782–2788
     [Google Scholar]
  23. Poyart C., Trieu-Cuot P. 1994; Heterogeneric conjugal transfer of the pheromone-responsive plasmid pIP964 (IncHlyI) of Enterococcus faecalis in the apparent absence of pheromone induction. FEMS Microbiol Lett 122:173–179
     [Google Scholar]
  24. Poyart C., Quesnes G., Trieu-Cuot P. 2000; Sequencing the gene encoding manganese-dependent superoxide dismutase for rapid species identification of enterococci. J Clin Microbiol 38:415–418
     [Google Scholar]
  25. van Schaik W., Top J., Riley D., Boekhorst J., Vrijenhoek J., Schapendonk C., Hendrickx A., Nijman I., Bonten M. other authors 2010; Pyrosequencing-based comparative genome analysis of the nosocomial pathogen Enterococcus faecium and identification of a large transferable pathogenicity island. BMC Genomics 11:239
     [Google Scholar]
  26. Wang L., Archer G. L. 2010; Roles of CcrA and CcrB in excision and integration of staphylococcal cassette chromosome mec , a Staphylococcus aureus genomic island. J Bacteriol 192:3204–3212
     [Google Scholar]
  27. Werner G., Willems R. J. L., Hildebrandt B., Klare I., Witte W. 2003; Influence of transferable genetic determinants on the outcome of typing methods commonly used for Enterococcus faecium . J Clin Microbiol 41:1499–1506
     [Google Scholar]
  28. Willems R. J., Top J., van Santen M., Robinson D. A., Coque T. M., Baquero F., Grundmann H., Bonten M. J. 2005; Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex. Emerg Infect Dis 11:821–828
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.041491-0
Loading
ccrABEnt serine recombinase genes are widely distributed in the Enterococcus faecium and Enterococcus casseliflavus species groups and are expressed in E. faecium
Microbiology 156, 3624 (2010); https://doi.org/10.1099/mic.0.041491-0
/content/journal/micro/10.1099/mic.0.041491-0
/content/journal/micro/10.1099/mic.0.041491-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error