clpB, a class III heat-shock gene regulated by CtsR, is involved in thermotolerance and virulence of Enterococcus faecalis
de Oliveira, Naira Elane Moreira and Abranches, Jaqueline and Gaca, Anthony O. and Laport, Marinella Silva and Damaso, Clarissa R. and Bastos, Maria do Carmo de Freire and Lemos, José A. and Giambiagi-deMarval, Marcia,, 157, 656-665 (2011),
doi = https://doi.org/10.1099/mic.0.041897-0,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Here, we transcriptionally and phenotypically characterized the clpB gene from Enterococcus faecalis. Northern blot analysis identified a monocistronic mRNA strongly induced at 48 and 50 °C. In silico analysis identified that the clpB gene encodes a protein of 868 aa with a predicted molecular mass of approximately 98 kDa, presenting two conserved ATP-binding domains. Sequence analysis also identified a CtsR-binding box upstream of the putative −10 sequence, and inactivation of the ctsR gene resulted in an approximately 2-log increase in clpB mRNA expression, confirming ClpB as a member of the CtsR regulon. While expression of clpB was induced by heat stress, a ΔclpB strain grew relatively well under many different stressful conditions, including elevated temperatures. However, expression of ClpB appears to play a major role in induced thermotolerance and in pathogenesis, as assessed by using the Galleria mellonella virulence model.,
language=,
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