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Volume 157, Issue 6

The role of the antimicrobial compound 2,4-diacetylphloroglucinol in the impact of biocontrol F113 on phytostimulators Free

Abstract

Pseudomonads producing the antimicrobial metabolite 2,4-diacetylphloroglucinol (Phl) can control soil-borne phytopathogens, but their impact on other plant-beneficial bacteria remains poorly documented. Here, the effects of synthetic Phl and Phl F113 on phytostimulators were investigated. Most strains were moderately sensitive to Phl. , Phl induced accumulation of carotenoids and poly-β-hydroxybutyrate-like granules, cytoplasmic membrane damage and growth inhibition in Cd. Experiments with F113 and a Phl mutant indicated that Phl production ability contributed to growth inhibition of Cd and Sp245. Under gnotobiotic conditions, each of the three strains, F113 and Cd and Sp245, stimulated wheat growth. Co-inoculation of Sp245 and resulted in the same level of phytostimulation as in single inoculations, whereas it abolished phytostimulation when Cd was used. Phl production ability resulted in lower cell numbers per root system (based on colony counts) and restricted microscale root colonization of neighbouring cells (based on confocal microscopy), regardless of the strain used. Therefore, this work establishes that Phl pseudomonads have the potential to interfere with phytostimulators on roots and with their plant growth promotion capacity.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • Ministère Français de la Recherche
© 2011 SGM
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2011-06-01
2024-04-24
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The role of the antimicrobial compound 2,4-diacetylphloroglucinol in the impact of biocontrol Pseudomonas fluorescens F113 on Azospirillum brasilense phytostimulators
Microbiology 157, 1694 (2011); https://doi.org/10.1099/mic.0.043943-0
/content/journal/micro/10.1099/mic.0.043943-0
/content/journal/micro/10.1099/mic.0.043943-0
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