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Volume 157, Issue 2

Fur regulation of the capsular polysaccharide biosynthesis and iron-acquisition systems in CG43 Free

Abstract

The ferric uptake regulator Fur has been reported to repress the expression of , a regulatory gene for the mucoid phenotype, leading to decreased capsular polysaccharide (CPS) biosynthesis in CG43. Here, quantitative real-time PCR (qRT-PCR) analyses and electrophoretic mobility shift assays showed that Fur also repressed the expression of the CPS regulatory genes and . Interestingly, deletion of or but not from the Δ strain was able to suppress the deletion effect of Fur. The availability of extracellular iron affected the amount of CPS, suggesting that Fur regulates CPS biosynthesis in an Fe(II)-dependent manner. Increased production of siderophores was observed in the Δ strain, suggesting that uptake of extracellular iron in is regulated by Fur. Fur titration assays and qRT-PCR analyses demonstrated that at least six of the eight putative iron-acquisition systems, identified by a search in the contig database of CG43, were directly repressed by Fur. We conclude that Fur has a dual role in the regulation of CPS biosynthesis and iron acquisition in .

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): 2CS, two-component system , CAS, chrome azurol S , CPS, capsular polysaccharide , Dip, 2,2-dipyridyl , EMSA, electrophoretic mobility shift assay , FURTA, Fur titration assay and qRT-PCR, quantitative real-time PCR
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2011-02-01
2024-04-18
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Fur regulation of the capsular polysaccharide biosynthesis and iron-acquisition systems in Klebsiella pneumoniae CG43
Microbiology 157, 419 (2011); https://doi.org/10.1099/mic.0.044065-0
/content/journal/micro/10.1099/mic.0.044065-0
/content/journal/micro/10.1099/mic.0.044065-0
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