RT Journal Article SR Electronic(1) A1 Ohbayashi, Takehisa A1 Irie, Atsushi A1 Murakami, Yoji A1 Nowak, Magdalena A1 Potempa, Jan A1 Nishimura, Yasuharu A1 Shinohara, Masanori A1 Imamura, TakahisaYR 2011 T1 Degradation of fibrinogen and collagen by staphopains, cysteine proteases released from Staphylococcus aureus JF Microbiology, VO 157 IS 3 SP 786 OP 792 DO https://doi.org/10.1099/mic.0.044503-0 PB Microbiology Society, SN 1465-2080, AB Staphylococcus aureus is the most frequently isolated pathogen in Gram-positive sepsis often complicated by a blood clotting disorder, and is the leading cause of infective endocarditis induced by bacterial destruction of endocardial tissues. The bacterium secretes cysteine proteases referred to as staphopain A (ScpA) and staphopain B (SspB). To investigate virulence activities of staphopains pertinent to clotting disorders and tissue destruction, we examined their effects on collagen, one of the major tissue components, and on plasma clotting. Both staphopains prolonged the partial thromboplastin time of plasma in a dose- and activity-dependent manner, with SspB being threefold more potent than ScpA. Staphopains also prolonged the thrombin time of both plasma and fibrinogen, indicating that these enzymes can cause impaired plasma clotting through fibrinogen degradation. Whereas SspB cleaved the fibrinogen Aα-chain at the C-terminal region very efficiently, ScpA degraded it rather slowly. This explains the superior ability of the former enzyme to impair fibrinogen clottability. Enzymically active staphopains, at concentrations as low as 10 nM, degraded collagen with comparable efficiency. These results show novel virulence activities of staphopains in degrading fibrinogen and collagen, and suggest an involvement of staphopains in the clotting impairment and tissue destruction caused by staphylococcal infection., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.044503-0