@article{mbs:/content/journal/micro/10.1099/mic.0.046268-0, author = "Cocotl-Yañez, Miguel and Sampieri, Arístides and Moreno, Soledad and Núñez, Cinthia and Castañeda, Miguel and Segura, Daniel and Espín, Guadalupe", title = "Roles of RpoS and PsrA in cyst formation and alkylresorcinol synthesis in Azotobacter vinelandii", journal= "Microbiology", year = "2011", volume = "157", number = "6", pages = "1685-1693", doi = "https://doi.org/10.1099/mic.0.046268-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.046268-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = " Azotobacter vinelandii is a soil bacterium that undergoes differentiation to form cysts that are resistant to desiccation. Upon induction of cyst formation, the bacterium synthesizes alkylresorcinols that are present in cysts but not in vegetative cells. Alternative sigma factors play important roles in differentiation. In A. vinelandii, AlgU (sigma E) is involved in controlling the loss of flagella upon induction of encystment. We investigated the involvement of the sigma factor RpoS in cyst formation in A. vinelandii. We analysed the transcriptional regulation of the rpoS gene by PsrA, the main regulator of rpoS in Pseudomonas species, which are closely related to A. vinelandii. Inactivation of rpoS resulted in the inability to form cysts resistant to desiccation and to produce cyst-specific alkylresorcinols, whereas inactivation of psrA reduced by 50 % both production of alkylresorcinols and formation of cysts resistant to desiccation. Electrophoretic mobility shift assays revealed specific binding of PsrA to the rpoS promoter region and that inactivation of psrA reduced rpoS transcription by 60 %. These results indicate that RpoS and PsrA are involved in regulation of encystment and alkylresorcinol synthesis in A. vinelandii.", }