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Volume 157, Issue 7

Characterization of the conjugative transposon CTnPg1: determination of the integration site and the genes essential for conjugal transfer Free

Abstract

In our previous study, extensive genomic rearrangements were found in two strains of the Gram-negative anaerobic bacterium () , and most of these rearrangements were associated with mobile genetic elements such as insertion sequences and conjugative transposons (CTns). CTnPg1, identified in strain ATCC 33277, was the first complete CTn reported for the genus In the present study, we found that CTnPg1 can be transferred from strain ATCC 33277 to another strain, W83, at a frequency of 10 to 10. The excision of CTnPg1 from the chromosome in a donor cell depends on an integrase (Int; PGN_0094) encoded in CTnPg1, whereas CTnPg1 excision is independent of PGN_0084 (a DNA topoisomerase I homologue; Exc) encoded within CTnPg1 and (PGN_1057) on the donor chromosome. Intriguingly, however, the transfer of CTnPg1 between strains requires RecA function in the recipient. Sequencing analysis of CTnPg1-integrated sites on the chromosomes of transconjugants revealed that the consensus attachment () sequence is a 13 bp sequence, TTTTCNNNNAAAA. We further report that CTnPg1 is able to transfer to two other bacterial species, and In addition, CTnPg1-like CTns are located in the genomes of other oral anaerobic bacteria, , and , with the same consensus sequence. These results suggest that CTns in the CTnPg1 family are widely distributed among oral anaerobic Gram-negative bacteria found in humans and play important roles in horizontal gene transfer among these bacteria.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • Ministry of Education, Culture, Sports, Science and Technology of Japan (Award 18018032 and 20592142)
  • Global COE Program at Nagasaki University
© 2011 SGM
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2011-07-01
2024-04-23
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Characterization of the Porphyromonas gingivalis conjugative transposon CTnPg1: determination of the integration site and the genes essential for conjugal transfer
Microbiology 157, 2022 (2011); https://doi.org/10.1099/mic.0.047803-0
/content/journal/micro/10.1099/mic.0.047803-0
/content/journal/micro/10.1099/mic.0.047803-0
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