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Volume 157, Issue 9

Differential response of towards friend and foe in mixed-species cultures Free

Abstract

In the oral biofilm, the ‘mitis’ streptococci are among the first group of organisms to colonize the tooth surface. Their proliferation is thought to be an important factor required for antagonizing the growth of cariogenic species such as . In this study, we used a three-species mixed culture to demonstrate that another ubiquitous early colonizing species, can greatly affect the outcome of the competition between a pair of antagonists such as and . Transcriptome analysis further revealed that responds differentially to its friend () and foe (). In the mixed culture with , all but one of the sugar uptake and metabolic genes were downregulated, while genes for alternative energy source utilization and HO tolerance were upregulated, resulting in a slower but persistent growth. In contrast, when cultured with , grew equally well or better than in monoculture and exhibited relatively few changes within its transcriptome. When was introduced into the mixed culture of and , it rescued the growth inhibition of . In this three-species environment, increased the expression of genes required for the uptake and metabolism of minor sugars, while genes required for oxidative stress tolerance were downregulated. We conclude that the major factors that affect the competition between and are carbohydrate utilization and HO resistance. The presence of in the tri-species culture mitigates these two major factors and allows to proliferate, despite the presence of .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • NIH (Award R15 DE019940)
  • Centers of Biomedical Research Excellence
  • COBRE (Award P20-RR018741-05)
© 2011 SGM
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2011-09-01
2024-04-20
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Differential response of Streptococcus mutans towards friend and foe in mixed-species cultures
Microbiology 157, 2433 (2011); https://doi.org/10.1099/mic.0.048314-0
/content/journal/micro/10.1099/mic.0.048314-0
/content/journal/micro/10.1099/mic.0.048314-0
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