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Volume 157, Issue 9

S-layer protein inhibition of -induced reorganization of the cytoskeleton and activation of MAPK signalling pathways in Caco-2 cells Free

Abstract

Surface layer (S-layer) proteins are crystalline arrays of proteinaceous subunits that are present as the outermost component of the cell wall in several species. The S-layer proteins have been shown to play a role in the antimicrobial activity of certain lactobacilli. However, it is not fully understood how the S-layer proteins exert this biological function. The aim of this study was to test the hypothesis that S-layer proteins antagonize Typhimurium (. Typhimurium) infection by protecting against F-actin cytoskeleton rearrangements and the activation of mitogen-activated protein kinase (MAPK) signalling pathways. Monolayer transepithelial electrical resistance (TER) was measured after . Typhimurium infection in Caco-2 cultured human intestinal cells with S-layer proteins. F-actin rearrangement and MAPK activation were also assessed by immunofluorescence staining or Western blotting. The results showed that when . Typhimurium was co-incubated with S-layer proteins, the Typhimurium-induced Caco-2 cell F-actin rearrangement was reduced, and the Typhimurium-induced TER decrease and interleukin 8 (IL-8) secretion were attenuated. Additionally, S-layer proteins could inhibit Typhimurium-induced phosphorylation of extracellular signal-regulated kinases 1 and 2 (ERK1/2), c-Jun amino-terminal kinase (JNK) and p38. This study indicates that S-layer proteins are able to inhibit . Typhimurium infection through blocking . Typhimurium-induced F-actin rearrangements and . Typhimurium-induced ERK1/2, JNK and p38 activation in Caco-2 cells. These data provide a rationale for the use of lactobacillus S-layer proteins as therapeutic and preventative agents, at least in infectious diarrhoea.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 30871858)
  • Program of Jiangsu Province in China (Award BE200830155)
© 2011 SGM
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2011-09-01
2024-04-16
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Lactobacillus S-layer protein inhibition of Salmonella-induced reorganization of the cytoskeleton and activation of MAPK signalling pathways in Caco-2 cells
Microbiology 157, 2639 (2011); https://doi.org/10.1099/mic.0.049148-0
/content/journal/micro/10.1099/mic.0.049148-0
/content/journal/micro/10.1099/mic.0.049148-0
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