RT Journal Article SR Electronic(1) A1 Chaudhuri, Roy R. A1 Yu, Lu A1 Kanji, Alpa A1 Perkins, Timothy T. A1 Gardner, Paul P. A1 Choudhary, Jyoti A1 Maskell, Duncan J. A1 Grant, Andrew J.YR 2011 T1 Quantitative RNA-seq analysis of the Campylobacter jejuni transcriptome JF Microbiology, VO 157 IS 10 SP 2922 OP 2932 DO https://doi.org/10.1099/mic.0.050278-0 PB Microbiology Society, SN 1465-2080, AB C ampylobacter jejuni is the most common bacterial cause of foodborne disease in the developed world. Its general physiology and biochemistry, as well as the mechanisms enabling it to colonize and cause disease in various hosts, are not well understood, and new approaches are required to understand its basic biology. High-throughput sequencing technologies provide unprecedented opportunities for functional genomic research. Recent studies have shown that direct Illumina sequencing of cDNA (RNA-seq) is a useful technique for the quantitative and qualitative examination of transcriptomes. In this study we report RNA-seq analyses of the transcriptomes of C. jejuni (NCTC11168) and its rpoN mutant. This has allowed the identification of hitherto unknown transcriptional units, and further defines the regulon that is dependent on rpoN for expression. The analysis of the NCTC11168 transcriptome was supplemented by additional proteomic analysis using liquid chromatography-MS. The transcriptomic and proteomic datasets represent an important resource for the Campylobacter research community., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.050278-0