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Volume 158, Issue 1

Taxon-specific metagenomics of reveals a narrow community of opportunistic species that regulate each other’s development Open Access

Abstract

In this paper, we report on the diversity of the mycotrophic fungus (teleomorph Ascomycota, Dikarya) revealed by a taxon-specific metagenomic approach. We designed a set of genus-specific internal transcribed spacer (ITS)1 and ITS2 rRNA primers and constructed a clone library containing 411 molecular operational taxonomic units (MOTUs). The overall species composition in the soil of the two distinct ecosystems in the Danube floodplain consisted of 15 known species and two potentially novel taxa. The latter taxa accounted for only 1.5 % of all MOTUs, suggesting that almost no hidden or uncultivable species are present at least in these temperate forest soils. The species were unevenly distributed in vertical soil profiles although no universal factors controlling the distribution of all of them (chemical soil properties, vegetation type and affinity to rhizosphere) were revealed. experiments simulating infrageneric interactions between the pairs of species that were detected in the same soil horizon showed a broad spectrum of reactions from very strong competition over neutral coexistence to the pronounced synergism. Our data suggest that only a relatively small portion of species is adapted to soil as a habitat and that the interaction between these species should be considered in a screening for as an agent(s) of biological control of pests.

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© 2012 SGM
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2012-01-01
2024-04-19
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Taxon-specific metagenomics of Trichoderma reveals a narrow community of opportunistic species that regulate each other’s development
Microbiology 158, 69 (2012); https://doi.org/10.1099/mic.0.052555-0
/content/journal/micro/10.1099/mic.0.052555-0
/content/journal/micro/10.1099/mic.0.052555-0
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