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Volume 158, Issue 3

Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in K-12 Free

Abstract

Two of the three [NiFe]-hydrogenases (Hyd) of have a hydrogen-uptake function in anaerobic metabolism. While Hyd-2 is maximally synthesized when the bacterium grows by fumarate respiration, Hyd-1 synthesis shows a correlation with fermentation of sugar substrates. In an attempt to advance our knowledge on the physiological function of Hyd-1 during fermentative growth, we examined Hyd-1 activity and levels in various derivatives of K-12 MC4100 with specific defects in sugar utilization. MC4100 lacks a functional fructose phosphotransferase system (PTS) and therefore grows more slowly under anaerobic conditions in rich medium in the presence of -fructose compared with -glucose. Growth in the presence of fructose resulted in an approximately 10-fold increase in Hyd-1 levels in comparison with growth under the same conditions with glucose. This increase in the amount of Hyd-1 was not due to regulation at the transcriptional level. Reintroduction of a functional -encoded fructose PTS into MC4100 restored growth on -fructose and reduced Hyd-1 levels to those observed after growth on -glucose. Reducing the rate of glucose uptake by introducing a mutation in the gene encoding the cAMP receptor protein, or consumption through glycolysis, by introducing a mutation in phosphoglucose isomerase, increased Hyd-1 levels during growth on glucose. These results suggest that the ability to oxidize hydrogen by Hyd-1 shows a strong correlation with the rate of carbon flow through glycolysis and provides a direct link between hydrogen, carbon and energy metabolism.

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© 2012 SGM
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2012-03-01
2024-04-19
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Analysis of hydrogenase 1 levels reveals an intimate link between carbon and hydrogen metabolism in Escherichia coli K-12
Microbiology 158, 856 (2012); https://doi.org/10.1099/mic.0.056622-0
/content/journal/micro/10.1099/mic.0.056622-0
/content/journal/micro/10.1099/mic.0.056622-0
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