Reduction of the monounsaturated fatty acid content of Escherichia coli results in increased resistance to oxidative damage
Pradenas, Gonzalo A. and Paillavil, Braulio A. and Reyes-Cerpa, Sebastián and Pérez-Donoso, José M. and Vásquez, Claudio C.,, 158, 1279-1283 (2012),
doi = https://doi.org/10.1099/mic.0.056903-0,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Reactive oxygen species (ROSs) affect several macromolecules and cellular components in eukaryotic and prokaryotic cells. In this work, the effect of various ROS-generating compounds on the Escherichia coli membrane was studied. Membrane fatty acid profiles, oxidative damage levels and bacterial resistance to these toxicants were determined. Studies included wild-type cells as well as a strain exhibiting a modified monounsaturated fatty acid (MUFA) profile (accomplished by overexpressing the β-hydroxyacyl acyl carrier protein dehydratase-encoding gene, fabA). Levels of membrane MUFAs and oxidative damage markers decreased slightly upon toxicant exposure with a concomitant increase in cell resistance to these ROS-generating compounds. A direct relationship between MUFAs and lipid peroxidation was observed. The lower the MUFA the lower the peroxide levels, suggesting that MUFAs are targets for membrane lipid oxidation.,
language=,
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