1887

Abstract

is a porcine respiratory pathogen, well known as the aetiological agent of Glässer’s disease. comprises strains of different virulence, but the virulence factors of this bacterium are not well defined. A neuraminidase activity has been previously detected in , but the role of sialylation in the virulence of this bacterium has not been studied. To explore the relationship between sialic acid (Neu5Ac) and virulence, we assessed the distribution of genes involved in sialic acid metabolism in 21 strains from different clinical origins (including nasal and systemic isolates). The neuraminidase gene , together with CMP-Neu5Ac synthetase and sialyltransferase genes , and , were included in the study. Neuraminidase activity was found to be common in isolates, and the gene from 12 isolates was expressed in and further characterized. Sequence analysis showed that the NanH predicted protein contained the motifs characteristic of the catalytic site of sialidases. While an association between the presence of and the different origins of the strains was not detected, the gene was predominantly present in the systemic isolates, and was not amplified from any of the nasal isolates tested. Analysis of the lipooligosaccharide (LOS) from reference strains Nagasaki (virulent, ) and SW114 (non-virulent, ) showed the presence of sialic acid in the LOS from the Nagasaki strain, supporting the role of sialylation in the virulence of this bacterial pathogen. Further studies are needed to clarify the role of sialic acid in the pathogenicity of .

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2012-08-01
2024-03-28
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