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Volume 158, Issue 7

Autoinducer-2 influences interactions amongst pioneer colonizing streptococci in oral biofilms Free

Abstract

and are among the first bacterial species to colonize clean tooth surfaces. Both produce autoinducer-2 (AI-2): a family of inter-convertible cell–cell signal molecules synthesized by the LuxS enzyme. The overall aim of this work was to determine whether AI-2 alters interspecies interactions between DL1 and 34 within dual-species biofilms in flowing human saliva. Based upon AI-2 bioluminescence assays, DL1 produced more AI-2 activity than 34 in batch culture, and both were able to remove AI-2 activity from solution. In single-species, saliva-fed flowcell systems, 34 formed scant biofilms that were similar to the mutant. Conversely, DL1 formed confluent biofilms while the mutant formed architecturally distinct biofilms that possessed twofold greater biovolume than the wild-type. Supplementing saliva with 0.1–10 nM chemically synthesized AI-2 (csAI-2) restored the DL1 biofilm phenotype to that which was similar to the wild-type; above or below this concentration range, biofilms were architecturally similar to that formed by the mutant. In dual-species biofilms, DL1 was always more abundant than 34. Compared with dual-species, wild-type biofilms, the biovolume occupied by 34 was reduced by greater than sevenfold when neither species produced AI-2. The addition of 1 nM csAI-2 to the dual-species – mutant biofilms re-established the biofilm phenotype to resemble that of the wild-type pair. Thus, this work demonstrates that AI-2 can alter the biofilm structure and composition of pioneering oral streptococcal biofilms. This may influence the subsequent succession of other species into oral biofilms and the ecology of dental plaque.

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2012-07-01
2024-04-24
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Autoinducer-2 influences interactions amongst pioneer colonizing streptococci in oral biofilms
Microbiology 158, 1783 (2012); https://doi.org/10.1099/mic.0.057182-0
/content/journal/micro/10.1099/mic.0.057182-0
/content/journal/micro/10.1099/mic.0.057182-0
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