1887

Abstract

is the aetiological agent of porcine pleuropneumonia and is normally transmitted by aerosols and direct contact between animals. has traditionally been considered an obligate pathogen of pigs and its presence in the environment has yet to be investigated. Here, the presence of was detected in drinking water of pig farms in Mexico using a PCR specific for the RTX toxin gene, . The presence of in farm drinking water was confirmed by indirect immunofluorescence using an -specific polyclonal antibody and by fluorescent hybridization. Viable bacteria from the farm drinking water were detected using the Live/Dead Light stain. Additionally, viable was selected and isolated using the cAMP test and the identity of the isolated bacteria were confirmed by Gram staining, a specific polyclonal antibody and an -specific PCR. Furthermore, biofilms were observed by scanning electron microscopy in -positive samples. In conclusion, our data suggest that viable is present in the drinking water of swine farms and may use biofilm as a strategy to survive in the environment.

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2013-03-01
2024-03-28
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