RT Journal Article SR Electronic(1) A1 Kim, Seung Min A1 Lee, Dong Hwan A1 Choi, Sang HoYR 2012 T1 Evidence that the Vibrio vulnificus flagellar regulator FlhF is regulated by a quorum sensing master regulator SmcR JF Microbiology, VO 158 IS 8 SP 2017 OP 2025 DO https://doi.org/10.1099/mic.0.059071-0 PB Microbiology Society, SN 1465-2080, AB A single polar flagellum and motility are potential virulence factors of Vibrio vulnificus, a foodborne pathogen. In the present study, the functions of FlhF and regulatory characteristics of the flhF expression of V. vulnificus were investigated. A deletion mutation in flhF abolished motility, flagella formation and flagellin synthesis, and introduction of flhF in trans complemented the defects. The flhF mutant revealed decreased expression of the class III and IV flagella genes, indicating that FlhF is a key regulator for the flagellar biogenesis of V. vulnificus. The influence of global regulatory proteins on the expression of flhF was examined and SmcR, a LuxR homologue, was found to downregulate flhF expression at the transcriptional level. SmcR represses flhF expression only in the stationary phase of growth and exerts its effects by directly binding to the flhF promoter region. Finally, an SmcR binding site, centred at 22.5 bp upstream of the transcription start site, was identified by a DNase I protection assay. The combined results demonstrate that a quorum sensing master regulator SmcR influences the motility and flagellar biogenesis of V. vulnificus through modulating the expression of FlhF in a growth-phase-dependent manner., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.059071-0