@article{mbs:/content/journal/micro/10.1099/mic.0.067744-0, author = "Quereda, Juan J. and Pucciarelli, M. Graciela and Botello-Morte, Laura and Calvo, Enrique and Carvalho, Filipe and Bouchier, Christiane and Vieira, Ana and Mariscotti, Javier F. and Chakraborty, Trinad and Cossart, Pascale and Hain, Torsten and Cabanes, Didier and García-del Portillo, Francisco", title = "Occurrence of mutations impairing sigma factor B (SigB) function upon inactivation of Listeria monocytogenes genes encoding surface proteins", journal= "Microbiology", year = "2013", volume = "159", number = "Pt_7", pages = "1328-1339", doi = "https://doi.org/10.1099/mic.0.067744-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.067744-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Bacteria of the genus Listeria contain the largest family of LPXTG surface proteins covalently anchored to the peptidoglycan. The extent to which these proteins may function or be regulated cooperatively is at present unknown. Because of their unique cellular location, we reasoned that distinct LPXTG proteins could act as elements contributing to cell wall homeostasis or influencing the stability of other surface proteins bound to peptidoglycan. To test this hypothesis, we used proteomics to analyse mutants of the intracellular pathogen Listeria monocytogenes lacking distinct LPXTG proteins implicated in pathogen–host interactions, such as InlA, InlF, InlG, InlH, InlJ, LapB and Vip. Changes in the cell wall proteome were found in inlG and vip mutants, which exhibited reduced levels of the LPXTG proteins InlH, Lmo0610, Lmo0880 and Lmo2085, all regulated by the stress-related sigma factor SigB. The ultimate basis of this alteration was uncovered by genome sequencing, which revealed that these inlG and vip mutants carried loss-of-function mutations in the rsbS, rsbU and rsbV genes encoding regulatory proteins that control SigB activity. Attempts to recapitulate this negative selection of SigB in a large series of new inlG or vip mutants constructed for this purpose were, however, unsuccessful. These results indicate that inadvertent secondary mutations affecting SigB functionality can randomly arise in L. monocytogenes when using common genetic procedures or during subculturing. Testing of SigB activity could be therefore valuable when manipulating genetically L. monocytogenes prior to any subsequent phenotypic analysis. This test may be even more justified when generating deletions affecting cell envelope components.", }