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Volume 160, Issue 12

Editor's Choice Localization and expression of the -endopeptidase LytF are influenced by mutations in LTA synthases and glycolipid anchor synthetic enzymes Free

Abstract

LytF plays a principal role in cell separation through its localization at the septa and poles on the vegetative cell surface. In this study, we found that a mutation in a major lipoteichoic acid (LTA) synthase gene – – results in a considerable reduction in the σ-dependent transcription of . The transcription was also reduced in mutants that affected glycolipid anchor biosynthesis. Immunofluorescence microscopy revealed that both the numbers of cells expressing LytF and the LytF foci in these mutants were decreased. In addition, the transcriptional activity of was almost abolished in the double (), triple (), and quadruple () mutants during vegetative growth. Cell separation defects in these mutants were partially restored with artificial expression of LytF. Interestingly, when transcription was induced in the single or multiple mutants, LytF was localized not only at the septum, but also along the sidewall. The amounts of LytF bound to cell wall in the single () and double () mutants gradually increased as compared with that in the WT strain, and those in the triple () and quadruple mutants were almost similar to that in the double mutant. Moreover, reduction of the transcription and chained cell morphology in the mutant were completely restored with artificial induction of the gene. These results strongly suggest that LTA influences the temporal, σ-dependent transcription of and is an additional inhibitory component to the vegetative cell separation enzyme LytF.

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • Grants-in-Aid for Scientific Research (C) (Award 23580107 and 19580085)
  • the Hokuto Foundation for Bioscience
  • the Kurata Memorial Hitachi Science and Technology Foundation, the Nagase Science and Technology Foundation
  • the Research Foundation for the Electrotechnology of Chubu
  • the Institute for Fermentation, Osaka
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2014-12-01
2024-04-19
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Localization and expression of the Bacillus subtilisdl-endopeptidase LytF are influenced by mutations in LTA synthases and glycolipid anchor synthetic enzymes
Microbiology 160, 2639 (2014); https://doi.org/10.1099/mic.0.080366-0
/content/journal/micro/10.1099/mic.0.080366-0
/content/journal/micro/10.1099/mic.0.080366-0
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