RT Journal Article SR Electronic(1) A1 McNally, Alan A1 La Ragione, Roberto M. A1 Best, Angus. A1 Manning, Georgina A1 Newell, Diane G.YR 2007 T1 An aflagellate mutant Yersinia enterocolitica biotype 1A strain displays altered invasion of epithelial cells, persistence in macrophages, and cytokine secretion profiles in vitro JF Microbiology, VO 153 IS 5 SP 1339 OP 1349 DO https://doi.org/10.1099/mic.0.2006/000919-0 PB Microbiology Society, SN 1465-2080, AB Despite being classically defined as non-pathogenic, there is growing evidence that biotype 1A Yersinia enterocolitica isolates may be aetiological agents of disease in humans. In previous studies, a potential link between motility and the ability of biotype 1A strains to invade cultured epithelial cells was observed. In an attempt to further investigate this finding, a flagella mutant was constructed in a human faecal Y. enterocolitica biotype 1A isolate. The flagella mutation abolished the ability of the strain to invade cultured human epithelial cells, although adherence was not affected. The aflagellate mutant was also attenuated in its ability to survive within cultured macrophages, being cleared after 3 h, whilst the wild-type persisted for 24 h after infection. Examination of cytokine secretion by infected macrophages also suggested that the flagella of biotype 1A strains act as anti-inflammatory agents, decreasing production of tumour necrosis factor (TNF)-α whilst increasing secretion of interleukin (IL)-10. Preliminary studies using porcine in vitro organ culture (IVOC) tissue suggested that the flagella mutant was also attenuated in its ability to colonize intestinal tissue., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2006/000919-0