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Volume 153, Issue 1

Abundance and ecophysiology of spp., glycogen-accumulating organisms in full-scale wastewater treatment processes Free

Abstract

The activity of glycogen-accumulating organisms (GAOs) in enhanced biological phosphorus removal (EBPR) wastewater treatment plants has been proposed as one cause of deterioration of EBPR. Putative GAOs from the , spp. (including ), were studied in full-scale EBPR plants to determine their distribution, abundance and ecophysiology. Fluorescence hybridization (FISH) demonstrated that spp. were generally low in abundance; however, in one plant surveyed, Cluster 2 constituted 9 % of all . FISH combined with microautoradiography revealed that both Cluster 1 and Cluster 2 were capable of taking up a narrow range of substrates including acetate, propionate, pyruvate and glucose under anaerobic and aerobic conditions. Formate, butyrate, ethanol and several other substrates were not taken up. Cluster 2 demonstrated a phenotype consistent with the current metabolic model for GAOs – anaerobic assimilation of acetate and reduction to polyhydroxyalkanoates (PHA) using the glycolytic pathway, and aerobic consumption of PHA. Polyphosphate-accumulating organisms (PAOs, ‘ Accumulibacter phosphatis’) and other putative GAOs (‘ Competibacter phosphatis’) co-existed in two plants with Cluster 2 , but in both plants, the latter organisms were more abundant. Thus Cluster 2 can be relatively abundant and could be carbon competitors of PAOs and other GAOs in EBPR plants.

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  • Accepted:
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  • Published Online:
Keyword(s): CLSM, confocal laser scanning microscope , EBPR, enhanced biological phosphorus removal , FISH, fluorescence in situ hybridization , GAO, glycogen-accumulating organism , MAR, microautoradiography , PAO, polyphosphate-accumulating organism , PHA, polyhydroxyalkanoates , Pi, inorganic phosphorus , PolyP, polyphosphate , TCA, tricarboxylic acid and VFA, volatile fatty acid
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2007-01-01
2024-04-18
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Abundance and ecophysiology of Defluviicoccus spp., glycogen-accumulating organisms in full-scale wastewater treatment processes
Microbiology 153, 178 (2007); https://doi.org/10.1099/mic.0.2006/001032-0
/content/journal/micro/10.1099/mic.0.2006/001032-0
/content/journal/micro/10.1099/mic.0.2006/001032-0
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