1887

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Volume 153, Issue 4

response to acidity involves activation of glutathione synthesis Free

Abstract

CIAT899 displays intrinsic tolerance to acidity, and efficiently nodulates at low pH. By characterizing a mutant strain, glutathione has been previously demonstrated to be essential for tolerance to acid stress. The wild-type gene region has been cloned and its transcription profile has been characterized by using quantitative real-time PCR and transcriptional gene fusions. Activation of the gene under acid-stress conditions was demonstrated. is also induced by UV irradiation. Upstream from a putative promoter element and an inverted repeat sequence were identified, which are proposed to be involved in expression under neutral and acidic conditions, respectively. Gel retardation assays indicate that transcription in acid conditions may involve protein binding to an upstream regulatory region.

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Keyword(s): qRT-PCR, quantitative real-time reverse transcriptase PCR and ROS, reactive oxygen species
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2007-04-01
2024-04-19
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Rhizobium tropici response to acidity involves activation of glutathione synthesis
Microbiology 153, 1286 (2007); https://doi.org/10.1099/mic.0.2006/003483-0
/content/journal/micro/10.1099/mic.0.2006/003483-0
/content/journal/micro/10.1099/mic.0.2006/003483-0
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