Isolation and characterization of putative Pseudobutyrivibrio ruminis promoters
Schoep, Tobias D. and Gregg, Keith,, 153, 3071-3080 (2007),
doi = https://doi.org/10.1099/mic.0.2007/006502-0,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Novel plasmids were constructed for the analysis of DNA fragments from the rumen bacterium Pseudobutyrivibrio ruminis. Five previously unidentified promoters were characterized using a novel primer extension method to identify transcription start sites. The genes downstream of these promoters were not identified, and their activity in expression of genomic traits in wild-type P. ruminis remains putative. Comparison with promoters from this and closely related species revealed a consensus sequence resembling the binding motif for the RNA polymerase σ 70-like factor complex. Consensus −35 and −10 sequences within these elements were TTGACA and ATAATATA respectively, interspaced by 15–16 bp. The consensus for the −10 element was extended by one nucleotide upstream and downstream of the standard hexamer (indicated in bold). Promoter strengths were measured by reverse transcription quantitative PCR and β-glucuronidase assays. No correlation was found between the composition and context of elements within P. ruminis promoters, and promoter strength. However, a mutation within the −35 element of one promoter revealed that transcriptional strength and choice of transcription start site were sensitive to this single nucleotide change.,
language=,
type=