RT Journal Article SR Electronic(1) A1 Schoep, Tobias D. A1 Gregg, KeithYR 2007 T1 Isolation and characterization of putative Pseudobutyrivibrio ruminis promoters JF Microbiology, VO 153 IS 9 SP 3071 OP 3080 DO https://doi.org/10.1099/mic.0.2007/006502-0 PB Microbiology Society, SN 1465-2080, AB Novel plasmids were constructed for the analysis of DNA fragments from the rumen bacterium Pseudobutyrivibrio ruminis. Five previously unidentified promoters were characterized using a novel primer extension method to identify transcription start sites. The genes downstream of these promoters were not identified, and their activity in expression of genomic traits in wild-type P. ruminis remains putative. Comparison with promoters from this and closely related species revealed a consensus sequence resembling the binding motif for the RNA polymerase σ 70-like factor complex. Consensus −35 and −10 sequences within these elements were TTGACA and ATAATATA respectively, interspaced by 15–16 bp. The consensus for the −10 element was extended by one nucleotide upstream and downstream of the standard hexamer (indicated in bold). Promoter strengths were measured by reverse transcription quantitative PCR and β-glucuronidase assays. No correlation was found between the composition and context of elements within P. ruminis promoters, and promoter strength. However, a mutation within the −35 element of one promoter revealed that transcriptional strength and choice of transcription start site were sensitive to this single nucleotide change., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2007/006502-0